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目的:探讨原发性肾病综合征(PNS)患儿外周血单个核细胞(PBMC)核因子-κB(NF-κΒ)活性水平变化的意义。方法:对25例确诊PNS的患儿及20例正常儿童,各抽取静脉血3mL培养PBMC,平分成3管,分别为:仅单纯培养(培养组);加入刺激剂IL-1β后培养(IL-1β组);加入抑制剂四苯磺酰苯乙基氯甲基酮(TPCK)后培养(TPCK组)。用ELISA法检测核蛋白提取物NF-κB活性的OD值。结果:培养组PNS患儿NF-κB的OD值为0.407±0.154,正常儿童为0.199±0.036;PNS患儿和正常儿童IL-1β组NF-κB活性最高,分别为0.571±0.290和0.308±0.060;TPCK组NF-κB活性最低,分别为0.348±0.118和0.163±0.039。PNS患儿和正常儿童的培养组、IL-1β组及TPCK组的NF-κB活性相比较差异有显著性(P<0.05)。培养组、IL-1β组及TPCK组PNS患儿的NF-κB活性均显著高于正常儿童(P<0.05)。结论:NF-κB可能在PNS的发生发展中起重要作用,PNS患儿肾小球系膜细胞的炎症反应刺激NF-κΒ的活化,促进病情的进一步发展,NF-κΒ可作为肾病综合征干预治疗的目标之一。
Objective: To investigate the significance of the changes of nuclear factor-κB (NF-κB) activity in peripheral blood mononuclear cells (PBMC) of children with primary nephrotic syndrome (PNS). Methods: Twenty-five children with confirmed PNS and 20 normal children were enrolled in this study. PBMCs were drawn from 3 mL venous blood and divided into three tubes, which were divided into three groups: cultured group (culture group), IL- -1β group); TPCK group was added after adding inhibitor tetraphenylsulfonyl phenethylchloromethyl ketone (TPCK group). The OD value of nuclear protein extract NF-κB activity was detected by ELISA. Results: The OD value of NF-κB in cultured PNS children was 0.407 ± 0.154 and 0.199 ± 0.036 in normal children. The activity of NF-κB in children with PNS and normal children was the highest (0.571 ± 0.290 and 0.308 ± 0.060, respectively) The lowest activity of NF-κB in TPCK group was 0.348 ± 0.118 and 0.163 ± 0.039, respectively. PNS children and normal children’s culture group, IL-1β group and TPCK group NF-κB activity difference was significant (P <0.05). The NF-κB activity in cultured group, IL-1βgroup and TPCK group were significantly higher than those in normal children (P <0.05). Conclusion: NF-κB may play an important role in the occurrence and development of PNS. Inflammatory reaction of mesangial cells in PNS may stimulate the activation of NF-κB and promote the further development of the disease. NF-κB may be used as a nephrotic syndrome intervention One of the goals of treatment.