Objective:To investigate the feasibility and safety of achieving total enteroscopy by consecutive bidirectional double-balloon enteroscopy(DBE)procedures.Methods:The demographic data,indication,initial insertion route,examination time for each insertion and the entire procedure,total enteroscopy rate,diagnostic yield and adverse events of patients who attempted to achieve total enteroscopy by consecutive bidirectional DBE procedures from January 2014 to December 2019 were retrospectively analyzed.Results:A total of 189 patients were included,and the total enteroscopy rate was 87.3％.Initiating the DBE procedure via the retrograde approach as the initial insertion route achieved a higher total enterosocpy rate(90.9％vs.78.9％,P=0.023),with shorter overall examination time(134.2±36.2 vs.156.9±47.6 min,P=0.017)and shorter examination time for the opposite insertion route(23.8±19.9 vs.53.1±27.6 min,P=0.000)compared with anteograde approach as the initial insertion route.The overall diagnostic yield was 37.6％.The diagnostic yield for successfully achieving total enteroscopy was higher,when compared to the yield for not successfully achieving total enteroscopy(39.4％vs.25％,P=0.029).The overall rate of adverse events was 2.1％(4/189).There was no significant difference in adverse event rate between the overall examination time≥2 h group and＜2 h group(2.1％vs.2.0％,P=0.593).Conclusion:Consecutive bidirectional DBE procedure is an effective and safe strategy for achieving total enteroscopy with a considerable success rate.This may be a promising option and alternative to traditional methods,and helpful to more promptly establish a definite diagnosis.The retrograde approach,as the initial insertion route,is preferred in clinical practice.