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[目的]探讨藤梨根提取物对人食管癌Eca-109细胞生长和凋亡的调节作用。[方法]MTT比色法检测藤梨根正丁醇提取物及乙酸乙酯提取物在不同浓度及不同时间对Eca-109细胞生长的抑制作用;用TUNEL法检测藤梨根不同提取物对诱导肿瘤细胞凋亡效应。[结果]藤梨根正丁醇提取物及乙酸乙酯提取物对人食管癌Eca-109细胞的生长具有明显的抑制作用,并具有浓度依赖性和时间依赖性;其中正丁醇提取物对肿瘤细胞的抑制作用较乙酸乙酯提取物更为明显。两种提取物均能明显地诱导肿瘤细胞的凋亡。[结论]藤梨根提取物有较好的抗肿瘤作用,抑制肿瘤细胞增殖、诱导肿瘤细胞凋亡可能是藤梨根抗肿瘤作用的机制之一。
[Objective] To investigate the regulation effect of Tengli root extract on the growth and apoptosis of human esophageal cancer Eca-109 cells. [Method] MTT assay was used to detect the inhibitory effect of n-butanol extract and ethyl acetate extract of Eupatorium adenophorum on the growth of Eca-109 cells at different concentrations and different time. TUNEL method was used to detect the effects of different extracts Tumor cell apoptosis effect. [Result] The n-butanol extracts and ethyl acetate extracts of Tropaeollum root could obviously inhibit the growth of human esophageal cancer Eca-109 cells in a concentration-dependent and time-dependent manner. The n-butanol extracts The inhibitory effect of tumor cells is more obvious than ethyl acetate extract. Both extracts clearly induced tumor cell apoptosis. [Conclusion] The rattan root extract has good anti-tumor effect, inhibiting tumor cell proliferation and inducing tumor cell apoptosis may be one of the mechanisms of anti-tumor effect of ragweed root.