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背景:现代医学研究发现天麻(Gastrodiaelata Bl)具有明显地心肌保护和血管扩张作用,可用于冠心病、高血压的治疗,但其作用机制仍需明确。目的:观察天麻水煎剂对去甲肾上腺素、KCl预收缩血管反应的舒张作用,并分析其作用途径。设计:分组设计、离体动物组织对照实验。单位:川北医学院生理学教研室。材料:选用健康家兔12只,兔龄七、八个月,雌雄不拘,由川北医学院实验动物中心提供。实验所用药品天麻水煎剂由慧仁堂药店鉴定提取,将其配制成10%,20%,40%和80%浓度备用。β肾上腺素能受体阻断剂普萘洛尔为北京第二制药厂产品;一氧化氮合酶抑制剂左旋硝基精氨酸为Sigma公司产品;甲烯蓝、环氧酶抑制剂吲哚美辛为江苏太仓制药厂产品。方法:实验于2006-01/2007-01在川北医学院药物研究所完成。采用家兔离体主动脉平滑肌标本,以去甲肾上腺素(1×10-6mol/L)预收缩主动脉后给予1.0,2.0,4.0,8.0和16.0g/L天麻水煎剂观察其张力变化,并观察去除血管内皮、给予1×10-4mol/L左旋硝基精氨酸、1×10-5mol/L甲烯蓝、1×10-5mol/L吲哚美辛和1×10-5mol/L普萘洛尔对血管张力的影响;血管张力经张力换能器输入BL-410智能型生物信号处理系统进行处理。另外观察8g/L天麻水煎剂对无内皮细胞血管环NA和KCl的量效收缩反应。主要观察指标:离体血管张力。结果:天麻对血管环静息张力无明显影响,但不同剂量的天麻可使1×10-6mol/L去甲肾上腺素预收缩血管产生明显舒张(r=0.85,t=18.45,P<0.01)。去除血管内皮、1×10-4mol/L左旋硝基精氨酸或1×10-5mol/L甲烯蓝可减弱天麻的舒张作用,但吲哚美辛和普萘洛尔对其无影响。另外,8g/L天麻水煎剂对无内皮细胞血管环去甲肾上腺素和KCl的量效收缩反应明显降低,且去甲肾上腺素和KCl的PD2(-log游离药物半数有效浓度)分别由温育前(6.90±0.93)mol/L和(1.53±0.55)mmol/L降低为(5.61±0.70)mol/L和(1.10±0.25)mmol/L,(t=2.41,3.82,P<0.05)。结论:天麻水煎剂对主动脉的舒张是内皮依赖性的,并与内皮一氧化氮的释放有关;也可通过拮抗ROC和PDC通道,抑制Ca2+的内流和释放等机制有关。
BACKGROUND: Modern medical studies have found that gastrodiaelata Bl has significant myocardial protection and vasodilation and can be used for the treatment of coronary heart disease and hypertension, but its mechanism of action still needs to be clarified. OBJECTIVE: To observe the relaxation effect of the decoction of Gastrodia elata on the vasoreactivity of norepinephrine and KCl precontraction, and to analyze the effect of the decoction. Design: group design, control experiments of isolated animal tissues. Unit: Department of Physiology, North Sichuan Medical College. MATERIALS: Twelve healthy rabbits were selected. The rabbits were seven or eight months old, male or female, provided by the Experimental Animal Center of Northern Sichuan Medical College. The medicine used for the experiment, Gastrodia decoction, was extracted from the Huirentang pharmacy and was formulated into 10%, 20%, 40% and 80% concentrations for use. Β-adrenoceptor blocker propranolol is the product of Beijing No. 2 Pharmaceutical Factory; nitric oxide synthase inhibitor L-nitro-arginine is a product of Sigma; Methylene blue, cyclo-oxygenase inhibitor Meixin is a product of Jiangsu Taicang Pharmaceutical Factory. METHODS: The experiment was performed at the Institute of Materia Medica, Northern Sichuan Medical College from January 2006 to January 2007. The isolated rabbit aortic smooth muscle specimens were used to observe the tension changes after precontracting the aorta with norepinephrine (1×10-6 mol/L), followed by 1.0, 2.0, 4.0, 8.0 and 16.0 g/L of gastrodia elata decoction. , And observe the removal of vascular endothelium, administration of 1×10-4 mol/L L-nitro arginine, 1×10-5 mol/L methylene blue, 1×10-5 mol/L indomethacin and 1×10-5 mol The effect of /L propranolol on vascular tone; vascular tension was processed by a tension transducer input BL-410 intelligent biological signal processing system. In addition, the dose-dependent contraction response of 8g/L gastrodia elata decoction to non-endothelial cell annulus vascular rings NA and KCl was observed. MAIN OUTCOME MEASURES: Isolated vascular tone. RESULTS: Gastrodia elata showed no significant effect on resting tension of vascular rings, but different doses of gastrodin produced significant relaxation of precontracted norepinephrine at 1×10-6 mol/L (r=0.85, t=18.45, P<0.01). . Removal of vascular endothelium, 1×10 -4 mol/L L-nitro arginine or 1×10 -5 mol/L methylene blue can weaken the relaxation effect of gastrodia elata, but indomethacin and propranolol have no effect on it. In addition, the dose-dependent contractile response of 8g/L of Gastrodia elata decuminata to endothelial cells without vascular norepinephrine and KCl was significantly reduced, and noradrenaline and KCl of PD2 (-log free drug half effective concentration) were respectively (6.90±0.93) mol/L and (1.53±0.55) mmol/L were reduced to (5.61±0.70) mol/L and (1.10±0.25) mmol/L, (t=2.41, 3.82, P<0.05). Conclusion: The relaxation of the aorta by the decoction of Gastrodia elata is endothelium-dependent and is related to the release of nitric oxide from the endothelium. It can also be related to the mechanism of inhibition of ROC and PDC channels and inhibition of Ca2+ influx and release.