论文部分内容阅读
目的:观察大蒜有效部位及其组合物对人胃癌MKN45细胞杀伤的作用。方法:采用体外大鼠脾淋巴细胞增殖实验和MTT法测定细胞杀伤作用,筛选出具有免疫增强作用和细胞毒作用的大蒜有效部位进行组合,并对大蒜有效部位及其组合物对肿瘤细胞杀伤作用进行对比,运用正交试验筛选出大蒜3个有效部位联合应用对人胃癌MKN45细胞杀伤作用的最佳配比。结果:大蒜总多糖+大蒜总皂苷(2#+5#)对大鼠淋巴细胞增殖具有明显的促进作用,大蒜油、大蒜总多糖、大蒜总皂苷(1∶1∶1)的联合应用,其杀伤肿瘤细胞的作用(IC50 13.3μg.mL-1)明显优于同等剂量的2个有效部位组合及大蒜油的单独用药(IC50 35μg.mL-1)。对MKN45细胞杀伤作用的顺序为大蒜油>大蒜多糖>大蒜总皂苷,3个有效部位组合最佳配比为大蒜油∶大蒜多糖∶大蒜总皂苷1∶2∶1。结论:3个部位联合应用对肿瘤细胞的杀伤作用最强,并且组合物最大优势是在扶正、祛邪的同时,可能减少大蒜油的刺激性。
Objective: To observe the effective part of garlic and its composition on human gastric cancer MKN45 cell killing. Methods: The cytotoxic effect of spleen lymphocyte proliferation in vitro and MTT method were assayed, and the effective parts of garlic with immune enhancement and cytotoxicity were screened out. The effective parts of garlic and its components were used to kill tumor cells The optimal ratio of 3 effective parts of garlic against MKN45 cells was screened by orthogonal test. Results: Total polysaccharide of garlic and total saponin of garlic (2 # + 5 #) could obviously promote the proliferation of rat lymphocytes. The combination of garlic oil, total polysaccharide of garlic and total saponin of garlic (1: 1: 1) The effect of killing tumor cells (IC50 13.3μg.mL-1) was significantly better than the same dose of 2 effective combination of parts and garlic oil alone (IC50 35μg.mL-1). The order of killing effect on MKN45 cells was garlic oil> garlic polysaccharide> total saponin of garlic, and the best combination of the three effective parts was garlic oil: garlic polysaccharide: garlic total saponin 1: 2: 1. Conclusion: The combined application of the three parts of the strongest killing effect on tumor cells, and the biggest advantage of the composition is the righting, Quxie at the same time, may reduce the irritation of garlic oil.