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在调查陕西、甘肃两省禾谷类三种病毒病:小麦线条花叶病毒(WSMV)病、小麦丛矮病毒(WRSV)病和玉米粗缩病毒(MRDV)病的发生危害基础上,采用超薄切片和电镜技术对发生在上述两省内的三种病毒病进行了病毒粒子的直接观察。结果表明:WSMV病毒粒子为线状,大小为611-871nm×l0(-11)nm,受侵细胞中有风轮状内含体.WRSV病毒粒子呈杆菌状或弹状,大小为268-436nm×37-57nm,弹状粒子外有一层套膜。MRDV病毒粒子为球形颗粒,具有双层衣壳,大小为65-71nm。电镜观察结果进一步明确了它们的病原特性及有关病理变化,所获得的病毒照片为西北地区禾谷类病毒的发生和鉴定提供了直接证据。
Based on the investigation of the occurrence of cereal three kinds of virus diseases in Shaanxi and Gansu Provinces such as wheat line mosaic virus (WSMV), wheat cluster stunt virus (WRSV) and maize rough virus (MRDV) Slicing and electron microscopy of virus particles occurred in the two provinces in the direct observation of the virus particles. The results showed that the WSMV virions were linear and ranged in size from 611 to 871 nm × 10 (-11) nm. WRSV virus particles bacilliform or bullet-shaped, the size of 268-436nm × 37-57nm, outside the elastic particles have a layer of mantle. The MRDV virions are spherical particles with a double-shell size of 65-71 nm. Electron microscopy results further clarify their pathogenicity and pathological changes, the obtained virus photos provide direct evidence for the occurrence and identification of cereal viruses in the northwestern region.