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目的通过测定颞叶内侧型癫痫大鼠模型海马的GABAA受体的三个亚单位α1β3γ2的mRNA表达水平,来探讨它们在该病的发病机制和发展过程中的作用,为癫痫的病因学研究和临床治疗提供可能的理论依据。方法用锂和匹罗卡品腹腔注射20只成年大鼠,制作颞叶内侧型癫痫大鼠模型。用断头法完整采集大鼠海马,RT-PCR技术扩增三个亚单位α1β3γ2的mRNA,通过t-检验与正常大鼠进行对比。结果实验组有16只大鼠制作癫痫模型成功,与β-actin电泳条带比值:α1(0.369±0.164),β3(0.499±0.273)γ2(0.429±0.346);对照组分别为α1(0.518±0.212),β3(0.291±0.116),γ2(0.231±0.132)。t-检验结果P<0.05,存在统计学差异。结论实验组大鼠GABAA受体可能发生了重组,它的三个亚单位α1β3γ2可能参与了颞叶内侧型癫痫的发病机制。
OBJECTIVE: To investigate the mRNA expression of the three subunits α1β3γ2 in the hippocampus of the medial temporal lobe epilepsy rat model to explore their roles in the pathogenesis and development of the disease. To study the etiology of epilepsy and Clinical treatment provides a possible theoretical basis. Methods 20 adult rats were injected intraperitoneally with lithium and pilocarpine to make the model of temporal lobe medial epilepsy. The hippocampus of rats were collected by decapitation method. The mRNA of three subunits α1β3γ2 was amplified by RT-PCR. Compared with normal rats by t-test. Results In the experimental group, 16 rats were successfully made epilepsy model. The ratios of α1 (0.369 ± 0.164) and β3 (0.499 ± 0.273) γ2 (0.429 ± 0.346) in control group were α1 (0.518 ± 0.212), β3 (0.291 ± 0.116), γ2 (0.231 ± 0.132). t-test results P <0.05, there is a statistical difference. Conclusion GABAA receptor may be reorganized in experimental group, and its three subunits α1β3γ2 may be involved in the pathogenesis of medial temporal lobe epilepsy.