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DNA纳米结构具有优异的生物相容性和高效的载药能力等性质,在生命科学和医学领域有很好的应用前景.然而,结构简单、分子量小的单链DNA(single strand DNA,ssDNA)进入细胞内的效率很低,且很快就被酶降解,无法完成载药任务.针对这种现状,本课题组通过DNA的自组装,将相互杂交的单链DNA构建为六螺旋(six-helix)结构,并对这种结构的稳定性和不同细胞的摄取情况进行了研究,发现与单链DNA相比,DNA六螺旋结构的稳定性更好,细胞摄取效率大幅提高,是一种潜在的药物载体.
DNA nanostructures have excellent biocompatibility and high drug-loading ability, and have good application prospects in the fields of life science and medicine.However, single-stranded DNA (ssDNA), which has a simple structure and a small molecular weight, Into the cells of the efficiency is very low, and soon was degraded by enzymes, can not complete the task of drug loading.In view of this situation, our group by DNA self-assembly, the hybridization of single-stranded DNA into six- helix) structure, and the stability of this structure and the uptake of different cells were studied and found that compared with single-stranded DNA, DNA six-helix structure is better, a substantial increase in cell uptake efficiency is a potential Of the drug carrier.