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本文研究目的在于评价影响接种物免疫能力的各种条件。实验用伯氏疟原虫K173株接种于幼龄的瑞士小鼠或C_3H/stz小鼠,每只小鼠自腹腔注射0.2毫升含10~5～5×10~7个感染疟原虫的红细胞。免疫接种后0～5天开始在饮水中加入300毫克/升浓度的磺胺嘧啶钠,连续21～33天进行抑制性治疗。停药后2天,每只免疫鼠用10~5感染疟原虫的红细胞进行攻击。免疫接种物的制备前,先自腹腔注射250国际单位肝素于供血小鼠,约经2～5分钟后放血,离心弃去血清及淡黄色层后加于含20%胎牛血清及5单位肝素的盐水或磷酸盐缓冲盐水的199培养基。含虫
The purpose of this study is to evaluate the conditions that affect the immunity of inoculum. Experimental P. berghei K173 strain was inoculated into young Swiss mice or C_3H / stz mice. Each mouse was injected intraperitoneally with 0.2 ml erythrocytes containing 10 ~ 5 ~ 5 × 10 ~ 7 plasmodium. 0-5 days after immunization began adding 300 mg / L sodium sulfadiazine in drinking water for 21 to 33 days for suppressive therapy. Two days after discontinuation, each immunized rat was challenged with 10 to 5 erythrocytes infected with malaria parasite. Prior to the preparation of the immunization, 250 IU heparin was injected intraperitoneally into the donor mice about 2 to 5 minutes after the injection of blood and centrifuged to remove the serum and light yellow layer and then added to 20% fetal bovine serum and 5 units of heparin Saline or phosphate buffered saline 199 medium. Containing insects