论文部分内容阅读
目的 研究胰腺癌、癌旁上皮和慢性胰腺炎组织中Smad4mRNA ,PTENmRNA ,P73mRNA表达特征及临床病理意义。方法 石蜡包块切片组织原位杂交染色法。结果 2 0例慢性胰腺炎导管上皮和腺泡上皮Smad4mRNA ,PTENmRNA均为阳性表达 ,但P73mRNA均为阴性表达 ;2 5例胰癌旁上皮仅 1例重度不典型增生者呈Smad4mRNA ,PTENmRNA阴性表达和P73mRNA阳性表达 (均为同一病例 ) ;5 3例胰腺癌Smad4mRNA ,PTENmRNA ,P73mRNA阳性病例分别为 34例 (6 4 % )、31例 (5 8% )和 2 7例 (5 1% ) ;胰腺癌Smad4mRNA ,PTENmRNA阳性率明显低于慢性胰腺炎 (χ2 =9 6 ,P <0 0 0 5 ;χ2 =11 8,P <0 0 0 5 )和癌旁上皮 (χ2 =9 0 ,P <0 0 0 5 ;χ2 =11 4 ,P <0 0 0 5 ) ;而P73mRNA阳性率明显高于慢性胰腺炎 (χ2 =15 2 ,P <0 0 0 5 )和癌旁上皮 (χ2 =16 2 ,P <0 0 0 5 )。高分化腺癌和未转移病例Smad4mRNA ,PTENmRNA阳性率明显高于低分化腺癌 (χ2 =4 4 ,P <0 0 5 ;χ2 =4 7,P <0 0 5 )和转移癌 (χ2 =4 2 ,P <0 0 5 ;χ2 =3 9,P <0 0 5 ) ;而P73mRNA阳性率明显低于低分化腺癌 (χ2 =6 4 ,P <0 0 2 5 )和转移癌 (χ2 =4 7,P <0 0 5 )。Smad4mRNA与PTEmRNA在胰腺癌中表达呈正相关 (χ2 =5 6 ,P <0 0 2 5 ) ,P73mRNA与Sma
Objective To study the expression of Smad4 mRNA, PTEN mRNA and P73 mRNA in pancreatic cancer, paracarcinoma and chronic pancreatitis and their clinicopathological significance. Methods paraffin - embedded tissue section in situ hybridization staining method. Results Twenty cases of chronic pancreatitis showed positive expression of Smad4 mRNA and PTEN mRNA in ductal epithelium and acinar epithelium but negative expression of P73 mRNA. Only one case of severe atypical hyperplasia of 25 cases of pancreatic carcinoma showed Smad4 mRNA and PTEN mRNA negative expression (P <0.05). The positive rates of Smad4 mRNA, PTEN mRNA and P73 mRNA in 53 pancreatic adenocarcinomas were 34 cases (64%), 31 cases (58%) and 27 cases The positive rates of Smad4 mRNA and PTEN mRNA were significantly lower than those of chronic pancreatitis (χ2 = 96, P <0 05; χ2 = 11 8, P 0 05) The positive rate of P73 mRNA was significantly higher than that of chronic pancreatitis (χ2 = 15 2, P <0 0 05) and adjacent para-epithelium (χ2 = 16 2, P 0 05) P <0 0 0 5). The positive rates of Smad4 mRNA and PTEN mRNA in well-differentiated adenocarcinoma and non-metastatic adenocarcinoma were significantly higher than those in poorly differentiated adenocarcinoma (χ2 = 4 4, P <0 05; χ2 = 47, P 0 05) and metastatic carcinoma 2, P 0 05; χ 2 = 39, P 0 05); while the positive rate of P73 mRNA was significantly lower than that of poorly differentiated adenocarcinoma (χ2 = 64, P 0 025) 4 7, P <0 0 5). There was a positive correlation between Smad4 mRNA and PTE mRNA expression in pancreatic cancer (χ2 = 56, P <0 025)