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研究不同混交比例对人工马尾松阔叶混交林土壤微生物群落功能多样性的影响,对石漠化地区植被恢复重建具有重要的意义。以贵阳市花溪区6个人工马尾松阔叶混交林为对象,采用Biolog-Eco技术,研究了0~20%(Ⅰ)、21%~40%(Ⅱ)、41%~60%(Ⅲ)、61%~80%(Ⅳ)、81%~100%(Ⅴ)这5种不同针阔混交比例下土壤微生物功能多样性。结果表明,平均颜色变化率(AWCD)表现为Ⅳ>Ⅱ2>Ⅴ>Ⅱ1>Ⅰ>Ⅲ,Ⅲ与Ⅱ、Ⅳ差异显著(p<0.05);土壤微生物量表现为Ⅲ>Ⅱ1>Ⅳ>Ⅴ>Ⅰ>Ⅱ2;Shannon-Wiener指数表现为Ⅳ>Ⅴ>Ⅱ2>Ⅱ1>Ⅰ=Ⅲ,Simpson优势度指数表现为Ⅳ>Ⅴ>Ⅱ2>Ⅱ1=Ⅲ>Ⅰ,Pielou均匀度指数表现为Ⅳ>Ⅴ>Ⅱ2>Ⅲ>Ⅰ>Ⅱ1,除Pielou均匀度指数外均表现为Ⅰ与Ⅴ差异显著(p<0.05)。主成分分析及聚类分析表明,Ⅳ、Ⅴ、Ⅱ2可归为一类,Ⅰ、Ⅱ1、Ⅲ归为另一类,阔叶树种比例的增加明显提升了马尾松林土壤微生物群落多样性,Ⅲ类样地土壤微生物多样性较低可能与土层薄、可利用基质较少有关;碳水化合物、多聚物、酚酸类3种碳源是不同混交比例的马尾松针阔混交林之间差异的敏感碳源。因此,混交比例的不同导致马尾松阔叶混交林土壤微生物群落功能多样性的差异。
Studying the influence of different mixed proportion on functional diversity of soil microbial community in Pinus massoniana-broadleaved mixed forest is of great significance to the restoration and reconstruction of vegetation in the rock desertification area. In this paper, 6 artificial Masson pine broad-leaved mixed forests in Huaxi District of Guiyang City were studied by Biolog-Eco technique. The effects of 0-20% (Ⅰ), 21% -40% (Ⅱ), 41% -60% , 61% -80% (Ⅳ) and 81% -100% (Ⅴ), respectively. The results showed that the average color change rate (AWCD) showed significant differences (p <0.05) for Ⅱ> Ⅱ> Ⅴ> Ⅱ1> Ⅰ> Ⅲ, Ⅲ and Ⅱ, Ⅳ and that of soil microbial biomass Ⅲ> Ⅱ1> Ⅳ> Ⅰ> Ⅱ 2, Shannon-Wiener index showed that Ⅳ> Ⅴ> Ⅱ 2> Ⅱ 1> Ⅰ = Ⅲ, Simpson dominance index showed that Ⅳ> Ⅴ> Ⅱ 2> Ⅱ2> Ⅲ> Ⅰ> Ⅱ 1, except Pielou evenness index showed significant difference between Ⅰ and Ⅴ (p <0.05). Principal component analysis and cluster analysis showed that Ⅳ, Ⅴ and Ⅱ2 could be classified into one group, Ⅰ, Ⅱ and Ⅲ belonged to another group, and the increase of broad-leaved tree species significantly enhanced the diversity of soil microbial community in Pinus massoniana forest. The lower soil microbial diversity may be related to the thin layer of soil and fewer available substrates. The three carbon sources of carbohydrate, polymer and phenolic acids are sensitive carbons with different ratios of mixed coniferous and broad-leaved mixed forests source. Therefore, the differences in the proportion of mixed plantation caused the difference in functional diversity of soil microbial community in the Pinus massoniana broadleaved mixed forest.