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目的:观察多发性硬化(MS)患者分泌γ-干扰素(IFN-γ)及白介素-10(IL-10)的细胞数的变化。方法:采用酶联免疫斑点技术比较MS患者、其他神经疾病患者和健康对照组外周血中髓鞘碱性蛋白(MBP)、乙酰胆碱受体(AChR)、植物血凝素(PHA)反应性及自发性分泌IFN-γ和IL-10的细胞数。结果:MS患者周围血单个核细胞中自发性分泌IFN-γ的细胞数和自发性分泌IL-10的细胞数多于其他神经系统疾病患者和健康对照组。经过MBP刺激后,MS患者分泌IFN-γ和IL-10的细胞数明显多于其他神经系统疾病患者和健康对照组。MS患者周围血细胞对MBP存在特异性反应。MS患者分泌IFN-γ的细胞数多于分泌IL-10的细胞数。结论:Th1类细胞因子(IFN-γ)分泌大于Th2类细胞因子(IL-10)与MS活动密切相关。MS患者的淋巴细胞对MBP存在特异性反应。
Objective: To observe the change of the number of IFN-γ and IL-10 secreted by patients with multiple sclerosis (MS). Methods: The levels of MBP, AChR, PHA in patients with MS, other neurological diseases and healthy controls were compared by enzyme-linked immunosorbent assay The number of cells secreting IFN-γ and IL-10. Results: The number of spontaneous IFN-γ-secreting cells and spontaneous IL-10 secreting cells in peripheral blood mononuclear cells of patients with MS were higher than those in other neurological diseases and healthy controls. After MBP stimulation, the number of MS patients secreting IFN-γ and IL-10 cells significantly more than other patients with neurological diseases and healthy controls. Peripheral blood cells of patients with MS specifically respond to MBP. MS patients have more IFN-γ secreting cells than IL-10 secreting cells. Conclusion: The secretion of Th1 cytokines (IFN-γ) is greater than Th2 cytokines (IL-10) and closely related to MS activity. Lymphocytes from MS patients have a specific response to MBP.