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以番茄(Solanum lycopericum)超表达桃SnRK1(蔗糖非发酵蛋白激酶–1)基因PpSnRK1α的株系及野生型为试材,研究在养分供应不足时SnRK1对植株生长的影响。结果表明,低营养条件下,转基因番茄叶片和根系中的Sn RK1酶活性比野生型高41.55%和39.46%;功能叶片的净光合速率平均比野生型高18.98%;低营养胁迫12 d的叶片SOD、POD、CAT活性比野生型高35.56%、28.85%和14.90%;根系活力比野生型高26.39%;茎和叶中氮磷含量显著高于野生型,钾含量两者差别不大,在根系中氮磷含量差别不大,而钾含量显著高于野生型,且氮素向地上部茎和叶中的分配比率增加。上述结果说明,在营养缺乏条件下,超表达PpSnRK1α可以提高番茄功能叶净光合速率,促进植株对氮素的吸收利用,从而延缓叶片衰老。
The effect of SnRK1 on plant growth was investigated when the nutrient supply was insufficient, using Solanum lycopericum overexpression line PmSnRK1α of peach SnRK1 (sucrose non-fermenting protein kinase-1) and wild type as test material. The results showed that the Sn RK1 enzyme activity in transgenic tomato leaves and roots was 41.55% and 39.46% higher than that in wild type plants under low nutrient conditions, and the average net photosynthetic rate of functional leaves was 18.98% higher than that of wild type. The leaves with low nutrient stress 12 d The activities of SOD, POD and CAT were 35.56%, 28.85% and 14.90% higher than those of wild type. The root activity was 26.39% higher than that of wild type. The contents of nitrogen and phosphorus in stem and leaves were significantly higher than those in wild type. The contents of nitrogen and phosphorus in roots were not significantly different, but the content of potassium was significantly higher than that in wild type, and the distribution ratio of nitrogen to shoots and leaves increased. The above results showed that in the condition of nutrient deficiency, overexpression of PpSnRK1α could increase the net photosynthetic rate of functional leaves of tomato and promote plant nitrogen uptake and utilization, thus retarding leaf senescence.