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通过根癌土壤杆菌介导法将外源抗虫基因导入4个陆地棉(GossypiumhirsutumL.)栽培品种。对转基因再生植株的移栽和嫁接研究发现,不同的定植方法对成活率和植株生长状态有显著的影响。直接移栽法、蛭石炼苗法和水培法的定植成活率均较低,缓苗时间也相对较长。而嫁接能够极大地提高再生植株的定植成活率并有效地缩短了缓苗时间,从而克服了棉花再生植株不易定植成活的困难,为基因工程乃至其他生物技术能够顺利地应用于棉花遗传改良奠定了基础。
Exogenous insect-resistant genes were introduced into four Gossypium hirsutum L. cultivars by Agrobacterium tumefaciens-mediated method. Transplanting transgenic plants and grafting study found that different methods of colonization on the survival rate and plant growth status have a significant impact. Direct transplanting method, vermiculite hardening method and hydroponics colonization survival rate are lower, Huanmiao time is relatively long. While grafting can greatly improve the survival rate of regenerated plants and effectively shorten the rehousing time so as to overcome the difficulty of colonization of cotton regenerated plants and lay the foundation for genetic engineering and other biotechnologies to be successfully applied to genetic improvement of cotton basis.