High water consumption and inefficient irrigation management in the agriculture sector of the middle and lower reaches of the Amu Darya River Basin (ADRB) have significantly influenced the gradual shrinking of the Aral Sea and its ecosystem. In this study, we investigated the crop water consumption in the growing seasons and the irrigation water requirement for different crop types in the lower ADRB during 2004–2017. We applied the FAO Penman–Monteith method to estimate reference evapotranspiration (ET0) based on daily climatic data collected from four meteorological stations. Crop evapotranspiration (ETc) of specific crop types was calculated by the crop coefficient. Then, we analyzed the net irrigation requirement (NIR) based on the effective precipitation with crop water requirements. The results indicated that the lowest monthly ET0 values in the lower ADRB were found in December (18.2 mm) and January (16.0 mm), and the highest monthly ET0 values were found in June and July, with similar values of 211.6 mm. The annual ETc reached to 887.2, 1002.1, and 492.0 mm for cotton, rice, and wheat, respectively. The average regional NIR ranged from 514.9 to 715.0 mm in the 10 Irrigation System Management Organizations (UISs) in the study area, while the total required irrigation volume for the whole region ranged from 4.2×109 to 11.6×109 m3 during 2004–2017. The percentages of NIR in SIW (surface irrigation water) ranged from 46.4％ to 65.2％ during the study period, with the exceptions of the drought years of 2008 and 2011, in which there was a significantly less runoff in the Amu Darya River. This study provides an overview for local water authorities to achieve optimal regional water allocation in the study area.