Objective:To assess the In vivo anlioxid Fanl and hepaloproleclive activity of metlianolic exlracl of Daucus carota(D.carota) seeds in experimental animals.Methods:Methanolic extracts of D.carota seeds is used for hepatoproleclion assessment.Oxidative stress were induced in rats by thioacetamide 100 nig/kg s.c.in four groups of rats(two test,standard and toxic control). Two test groups received D.carota seeds extract[DCSE) at doses of 200 mg/kg and 400 mg/kg. Standard group received silymarin(25 mg/kg) and toxic control received only thioacetamide. Control group received only vehicle.On the 8th day animals were sacrificed and liver enzyme like serum glutamic pyruvic transaminase(SGPT),serum glutamic-oxaloacetic transaminase(SCOT) and alkaline phosphatase(ALP)were estimated in blood serum and antioxidant enzyme like superoxide disnuituse(SOD),cululase(CAT),glutathione reductase(CKD),glutathione peroxidase(GPX),glutalhione-S-transferase(GST)and lipid peroxidation(LPO)were estimated in liver homogcnatc.Results:A significant decrease in SGPT,SCOT and ALP levels was observed in all drug treated groups as compared to thioacetamide group(P<0.001) and in case of antioxidant enzyme a significant(P<0.001) increase in SOD.CAT,GRD,GPX and GST was observed in all dmg treated groups as compared with thioacetamide group.But in case of LPO a significant(P <0.001) reduction was observed as compared to toxic control group.Conclusions:DCSE has contributed lo the reduction of oxidative stress and the protection of liver in experimental rals. Objective: To assess the In vivo anlioxidation Fanl and hepaloproleclive activity of metlianolic exlracl of Daucus carota (D. carota) seeds in experimental animals.Methods: Methanolic extracts of D.carota seeds is used for hepatoproleclion assessment. Oxidative stress were induced in rats by Two test groups received D.carota seeds extract (DCSE) at doses of 200 mg / kg and 400 mg / kg. Standard group received silymarin (25 mg / kg) and toxic control received only thioacetamide. Control group received only vehicle. On the 8th day animals were sacrificed and liver enzyme like serum glutamic pyruvic transaminase (SGPT), serum glutamic-oxaloacetic transaminase (SCOT) and alkaline phosphatase ALP) were estimated in blood serum and antioxidant enzymes like superoxide disnuituse (SOD), cululase (CAT), glutathione reductase (CKD), glutathione peroxidase (GPX), glutalhione-S-transferase in li Results of a significant decrease in SGPT, SCOT and ALP levels were observed in all drug treated groups as compared to thioacetamide group (P <0.001) and in case of antioxidant enzymes a significant (P <0.001) increase in SOD. CAT , GRD, GPX and GST was observed in all dmg treated groups as compared with thioacetamide group. But in case of LPO a significant (P <0.001) reduction was observed as compared to toxic control group. Conclusions: DCSE has contributed lo the reduction of oxidative stress and the protection of liver in experimental rals.