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目的 评价非那雄胺对大鼠前列腺微血管的影响并探讨其机制。方法 ①将雄性SD大鼠随机分为三组 ,每组 10只 ,按4 0mg/(kg·d)喂饲非那雄胺 7d(B组 )、14d(C组 ) ,以喂饲生理盐水为对照组 (A组 )。②HE染色观察采用墨汁灌注染色的大鼠前列腺微血管的改变。③免疫组织化学SP法检测大鼠前列腺组织中内皮型一氧化氮合酶 (eNOS)的蛋白表达。结果 ①B、C组的微血管密度较A组分别下降了 35 .2 %、5 6 %。②eNOS的蛋白表达较A组分别减少 15 .7%、5 2 .5 %。结论 非那雄胺可抑制大鼠前列腺组织中eNOS的蛋白表达进而抑制前列腺的微血管生成。
Objective To evaluate the effect of finasteride on rat prostatic microvessels and to explore its mechanism. Methods ① Male Sprague-Dawley rats were randomly divided into three groups, 10 rats in each group, fed with finasteride for 4 days (Group B) and 14 days (Group C) fed with 40 mg / (kg · d) For the control group (A group). (2) HE staining was used to observe the changes of prostatic microvessels in rats by ink perfusion staining. ③ Immunohistochemical SP method was used to detect the protein expression of endothelial nitric oxide synthase (eNOS) in rat prostatic tissue. Results ① The microvessel density of group B and C decreased by 35.2% and 56% respectively compared with that of group A. ② The protein expression of eNOS was 15.7% and 52.5% lower than that of group A respectively. Conclusion Finasteride can inhibit eNOS protein expression in rat prostate tissue and thus inhibit prostate angiogenesis.