利用改进的QuEChERS前处理方法提取鱼肉中依诺沙星(EO)、诺氟沙星(NRF)、盐酸环丙沙星(CPFHCl)和恩诺沙星(ERF)4种氟喹诺酮类药物。采用90%乙腈酸溶液作为提取剂,结合分散固相萃取(DSPE)净化,以PSA固体颗粒作吸附剂,利用高效液相色谱(HPLC)进行检测。4种药物回收率可达80%～96%,相对标准偏差均小于6%。结果证明此方法能够满足鱼肉中氟喹诺酮类兽药残留的简便、快速、高效检测。 Fluoroquinolones were extracted from fish flesh with enrofloxacin (EO), norfloxacin (NRF), ciprofloxacin hydrochloride (CPFHCl) and enrofloxacin (ERF) by a modified QuEChERS pretreatment method. A 90% acetonitrile solution was used as the extractant, combined with dispersive solid-phase extraction (DSPE) purification, PSA solid particles as adsorbent, and detection by high performance liquid chromatography (HPLC). The recoveries of four kinds of drugs can reach 80% ~ 96% with relative standard deviations less than 6%. The results show that this method can meet the simple, rapid and efficient detection of fluoroquinolone veterinary drug residues in fish.