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诊断早期胰腺癌用超声和放射线是相当困难的,加上胰腺癌和慢性胰腺炎在临床上很难鉴别。胰汁的细胞诊断胰腺癌有相当高特异性,有30—79％的敏感性但不能重复。作者用内窥镜检查收集纯胰汁,提取DNA,在胰腺癌中检出第12密码子上k-ras癌基因的点突变有极高发生率,并认识在癌形式中起着一种关键性作用。方法:利用多聚酶链反应(PCR)和限制酶消化法两者相结合的两步法检查k-ras点突变,随后用放射性同位素单链构型多态性(SSCP)分析。
Diagnosis of early pancreatic cancer using ultrasound and radiation is quite difficult, plus pancreatic cancer and chronic pancreatitis are difficult to identify clinically. Pancreatic cell diagnosis of pancreatic cancer is highly specific, with sensitivity of 30-79% but not repeatable. The author used endoscopy to collect pure pancreatic juice, extract DNA, and detect the point mutation of the k-ras oncogene in the 12th codon in pancreatic cancer, which has a very high incidence, and recognize that it plays a key role in the cancer form. Sexual function. METHODS: K-ras point mutations were examined using a two-step method combining polymerase chain reaction (PCR) and restriction enzyme digestion, followed by analysis of radioisotope single-stranded conformation polymorphism (SSCP).