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蛋白质的浓度可以由蛋白质的物理和化学性质的数据来推算。物理性质有折光率、比重、紫外吸收等;化学性质有含氮量、缩脲反应和福林——酚试剂反应等。目前,实验室一般常用的方法是紫外分光光度法、缩脲法、福林——酚试剂法等。这些方法各有优缺点,缩脲法是利用肽键和碱性Cu~(2+)的缩脲反应产生的蓝色化合物进行比色。它的颜色不受蛋白质中氨基酸组成的影响,但是灵敏度较差。福林——酚试剂的方法是利用蛋白质中酪氨酸和色氨酸在碱性溶液中易将磷钼酸和磷钨酸的混合物还原为蓝色化合
The concentration of protein can be deduced from the data of the physical and chemical properties of the protein. Physical properties of refractive index, specific gravity, UV absorption, etc .; chemical properties of nitrogen content, urea-urea reaction and reaction - phenolic reagents. At present, the common laboratory methods are UV spectrophotometry, urea-urea method, Fulin-phenol reagent method. Each of these methods has its own advantages and disadvantages. The urea-urea method is a colorimetric method using a blue compound produced by a peptide bond and a urea-urea reaction of basic Cu 2+. Its color is not affected by the amino acid composition of the protein, but the sensitivity is poor. The method of the Fulin-Phenol reagent is to utilize the tyrosine and tryptophan in the protein to easily reduce the mixture of phosphomolybdic acid and phosphotungstic acid into blue compound in an alkaline solution