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目的研究丝/苏氨酸激酶周期素依赖性激酶样-5(CDKL5)在胃癌组织中的表达,并分析其与胃癌患者的临床病理特征之间的关系。方法应用荧光实时定量PCR法和免疫印迹法分别检测45例胃癌及其癌旁组织中CDKL5 m RNA及蛋白的表达,然后用免疫荧光方法检测CDKL5蛋白在AGS和MKN-45胃癌细胞株中的表达情况。结果 CDKL5 m RNA和蛋白在胃癌组织中的表达均明显高于其在相应的癌旁组织中的表达(P<0.05);CDKL5 m RNA的表达与胃癌患者的年龄(P=0.033)、血管浸润(P=0.007)、T分期(P=0.049)和TNM分期(P=0.041)有关;多重逐步回归分析结果显示,血管浸润(P=0.013)和TNM分期(P=0.024)是影响CDKL5m RNA表达的重要因素;胃癌组织和细胞株中CDKL5蛋白除了在相对分子质量为107×103条带处显影外,还检测到在相对分子质量为85×103处的显影条带。结论 CDKL5在胃癌组织和细胞中均呈高表达,其m RNA表达与胃癌患者的血管浸润和TNM分期有关;CDKL5蛋白分别在相对分子质量为107×103和85×103条带处显影,提示CDKL5在蛋白水平可能存在不同的修饰方式或者表达方式。
Objective To investigate the expression of CDKL5 in gastric carcinoma and its relationship with the clinicopathological features of gastric cancer. Methods Real-time quantitative PCR and Western blotting were used to detect the expression of CDKL5 mRNA and protein in 45 cases of gastric cancer and its adjacent tissues respectively. Then the expression of CDKL5 protein in AGS and MKN-45 gastric cancer cell lines was detected by immunofluorescence Happening. Results The expression of CDKL5 mRNA and protein in gastric cancer tissues was significantly higher than that in corresponding adjacent tissues (P <0.05). The expression of CDKL5 mRNA was significantly correlated with the age (P = 0.033), vascular infiltration (P = 0.007), T stage (P = 0.049) and TNM stage (P = 0.041). The results of multiple stepwise regression analysis showed that vascular infiltration (P = 0.013) and TNM stage (P = 0.024) CDKL5 protein in gastric cancer tissue and cell lines in addition to the development of the molecular weight of 107 × 103 at the band, but also detected at a relative molecular mass of 85 × 103 at the development of the band. Conclusion The expression of CDKL5 in gastric cancer tissues and cells is highly correlated with the expression of m RNA in vascular infiltration and TNM staging in patients with gastric cancer. CDKL5 protein is developed at the relative molecular mass of 107 × 103 and 85 × 103, respectively, suggesting that CDKL5 There may be different ways of modification or expression at the protein level.