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目的 了解高脂饮食所致的大鼠胰岛素抵抗（ＩＲ）模型血及组织中肿瘤坏死因子－α（ＴＮＦ－α）含量的变化。方法 用脂肪占摄入热卡６１％ 的饮食饲养大鼠７周，以葡萄糖－胰岛素耐量试验（Ｇ－ＩｎｓＴＴ）筛选出ＩＲ大鼠，以放射免疫法分别检测其血清、肝脏、红色股四头肌、脂肪组织匀浆中ＴＮＦ－α的含量。结果 高脂饮食致ＩＲ大鼠血及组织中ＴＮＦ－α含量均明显升高，且其升高程度与高胰岛素血症呈正相关，与Ｇ－ＩｎｓＴＴ中的Ｋ值呈负相关。结论 高脂饮食的ＩＲ大鼠中存在ＴＮＦ－α合成和／或分泌的升高，且与ＩＲ的发生密切相关
Objective To investigate the changes of tumor necrosis factor-α (TNF-α) in blood and tissues of rats with insulin resistance (IR) induced by high-fat diet. Methods Fat rats were fed with a diet of 61% fat for 7 weeks, and IR rats were screened by G-InsTT. Serum, liver and red stock were detected by radioimmunoassay Muscle, adipose tissue homogenate TNF-α content. Results The levels of TNF-α in blood and tissues of IR rats induced by high-fat diet were significantly increased, and the levels of TNF-α were positively correlated with hyperinsulinemia and negatively correlated with K values of G-InsTT. Conclusions There is an increase in the synthesis and / or secretion of TNF-α in IR rats fed high-fat diet and is closely related to the occurrence of IR