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选择钥孔虫戚血蓝蛋白（ Ｋ Ｌ Ｈ）和牛血清白蛋白（ Ｂ Ｓ Ａ）作为半抗原伏马菌素 Ｂ１ （ Ｆ Ｂ１ ）的载体蛋白，以戊二醛一步法分别制备免疫原 Ｋ Ｌ Ｈ Ｆ Ｂ１ 和固相抗原 Ｂ Ｓ Ａ Ｆ Ｂ１ 蛋白结合物。用 Ｋ Ｌ Ｈ Ｆ Ｂ１ 免疫 Ｂａｌｂ／ｃ 小鼠后，应用细胞融合技术，筛选后获得了１株稳定分泌抗 Ｆ Ｂ１ 单克隆抗体的杂交瘤细胞株（ Ｆ Ｂ１ ４ Ｇ４ ），分泌的抗体属于 Ｉｇ Ｇ１ 亚类。腹水的抗体效价为１ｖ１×１０７ ，抗体与其它３种真菌毒素、 Ｆ Ｂ３ 和２种载体蛋白均无交叉反应，与 Ｆ Ｂ２有轻微的交叉反应。
Keyhole limpet hemocyanin (K L H) and bovine serum albumin (B S A) were selected as carrier proteins of haptens F B1. The immunogen K L H F B1 and solid phase antigen B S A F B1 protein conjugates. After Balb / c mice were immunized with KLH-F B1, a hybridoma cell strain (F B1 4 G4) stably secreting anti-F B1 monoclonal antibody was screened by the cell fusion technique. The secreted antibodies belonged to Ig G1 subclass. Ascites antibody titer of 1v1 × 107, antibody and other three kinds of mycotoxins, F B3 and two kinds of carrier protein cross-reaction, and F B2 have a slight cross-reaction.