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目的:探讨自发性高血压大鼠(Spontaneously hypertensive rats,SHR)肠系膜淋巴微循环并对其特点进行分析。方法:以Wistar大鼠为对照组,SHR为实验组,分别取10只雄性8周龄Wistar大鼠和8周龄SHR(SHR8W)大鼠,麻醉、固定并暴露肠系膜后,在微循环活体显微镜下观察肠系膜微静脉及微淋巴管,并利用Vas-Track自动跟踪测量系统对视频进行定量检测,比较分析自发性高血压大鼠肠系膜白细胞流变学、舒缩速度、舒缩幅度、舒缩时长比等参数的变化。结果:SHR肠系膜白细胞滚动速度明显增加,炎症反应集中体现在30-40μm的微静脉上,与同周龄的Wistar大鼠相比,SHR肠系膜微淋巴管自律运动的相对振幅显著性降低(31.70%±11.70%vs 43.30%±12.40%,P<0.05),舒张期的幅度、时长、传播速度分别受到显著性抑制(30.00±16.00μm,13.90±5.30μm/s,2.67±1.30 s versus 46.00±14.00μm,16.70±7.70μm/s,3.24±1.60 s,P<0.001,P<0.05,P<0.001),收缩期的幅度、时长分别下降至34.00±17.0μm、1.04±0.48 s,而与对照组相比,SHR收缩期传播速度代偿性增高(34.60±14.05μm/s vs 32.00±11.30μm/s,P<0.05)。结论:SHR肠系膜微淋巴管自律运动出现功能障碍;SHR肠系膜不同管径级别微静脉的分布是呈现不同的特点。
Objective: To investigate the mesenteric lymph microcirculation in spontaneously hypertensive rats (SHR) and analyze its characteristics. Methods: Wistar rats were used as control group and SHR was used as experimental group. Ten male Wistar rats of 8 weeks old and SHR8W rats of 8 weeks old were randomly divided into four groups: control group, fixed group and exposed mesentery group. The mesenteric venules and lymphatic vessels were observed, and the video was detected by Vas-Track automatic tracking system. The changes of mesenteric leukocyte rheology, systolic and diastolic velocity, systolic and diastolic velocity, systolic and diastolic time of spontaneously hypertensive rats Than the other parameters of change. RESULTS: The rolling velocity of mesenteric leukocytes was significantly increased in SHR and the inflammatory reaction was concentrated in the micro venules 30-40μm. Compared with Wistar rats of the same age, the relative amplitude of spontaneous movement of mesenteric lymphatic vessels in SHR was significantly decreased (31.70% ± 11.70% vs 43.30% ± 12.40%, P <0.05). The amplitude, duration and velocity of diastole were significantly inhibited (30.00 ± 16.00μm, 13.90 ± 5.30μm / s, 2.67 ± 1.30s versus 46.00 ± 14.00 The amplitude and duration of systolic phase decreased to 34.00 ± 17.0 μm and 1.04 ± 0.48 s, respectively, while those in the control group (P <0.05, P <0.001, P < Compared with SHR, systolic velocity was compensatory (34.60 ± 14.05μm / s vs 32.00 ± 11.30μm / s, P <0.05). CONCLUSIONS: There is dysfunction of spontaneous movement of mesenteric lymphatic vessels in SHR. The distribution of microvessels in different diameter classes of SHR mesentery presents different characteristics.