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目的:探讨Pokemon基因在肾细胞癌组织中的表达及其生物学意义。方法:收集47例肾癌手术切除的癌、癌旁及邻近正常组织,用逆转录实时聚合酶链反应(Real-time PCR)及蛋白质印迹法分别从核酸及蛋白水平检测标本中Pokemon基因的表达,并结合性别、年龄、肿瘤大小、病理分级和TNM分期等临床资料进行统计分析,对mRNA和蛋白表达的水平进行相关性研究。结果:Real-time PCR及蛋白质印迹法均检测出Pokemon在肾癌、癌旁及正常对照组织中的表达,差异有统计学意义,P<0.05。肾癌组织中,Pokemon的表达与肿瘤病理分级有关,而与患者的性别、年龄、肿瘤最大直径、TNM及病理类型无明显相关性。肾组织中,Pokemon的mRNA与蛋白的表达呈正相关,r=0.494,P<0.05。结论:Pokemon表达和肾癌的发病及恶性程度有关,有望成为肾癌的预后指标和肾癌靶向治疗的有效靶点。
Objective: To investigate the expression of Pokemon gene in renal cell carcinoma and its biological significance. Methods: Totally 47 cases of carcinoma of the kidney and adjacent normal tissues were collected. Real-time PCR and Western blotting were used to detect the expression of Pokemon gene from nucleic acid and protein level respectively. Combined with clinical data such as sex, age, tumor size, pathological grade and TNM stage, statistical analysis was carried out to study the correlation between mRNA and protein expression level. Results: The expression of Pokemon was detected by Real-time PCR and Western blot in renal cell carcinoma, adjacent normal tissues and normal control tissues, with statistical significance (P <0.05). In renal cell carcinoma, the expression of Pokemon was related to the pathological grade of tumor, but not to the patient’s sex, age, maximum diameter of tumor, TNM and pathological type. In renal tissues, Pokemon mRNA and protein expression was positively correlated, r = 0.494, P <0.05. Conclusion: The expression of Pokemon is related to the incidence and malignant degree of renal cell carcinoma, which may be a prognostic indicator of renal cell carcinoma and an effective target of targeted therapy of renal cell carcinoma.