论文部分内容阅读
目的采用HPLC测定芩暴红止咳胶囊中东莨菪内酯、黄芩苷、黄芩素、杜鹃素的含量。方法 Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm),流动相:甲醇(A)-1%冰醋酸溶液(B),梯度洗脱,流速:0.8 mL·min-1;检测波长:295 nm;柱温:30℃。结果东莨菪内酯线性范围为1.27~124.70μg,r=0.999 9,加样回收率为98.3%(RSD=1.1%);黄芩苷线性范围为8.07~538.20μg,r=1.000 0,样品加样回收率为98.0%(RSD=1.2%);黄芩素线性范围为1.12~111.60μg,r=1.000 0,加样回收率为98.5%(RSD=0.9%);杜鹃素线性范围为1.06~106.40μg,r=1.000 0,加样回收率为99.0%(RSD=1.4%)。结论本方法简便、准确,重复性好,专属性高,可用于制剂的质量控制。
Objective To determine the contents of scopoletin, baicalin, baicalein and farrellin in Qinzhenghongzhike Capsule by HPLC. Methods The mobile phase was eluted with Eclipse XDB-C18 column (4.6 mm × 250 mm, 5 μm) and the mobile phase consisted of methanol (A) -1% glacial acetic acid (B) with gradient elution at a flow rate of 0.8 mL · min -1. 295 nm; column temperature: 30 ° C. Results The linear range of scopoletin was 1.27 ~ 124.70μg, r = 0.999 9, the recovery was 98.3% (RSD = 1.1%). The linear range of baicalin was 8.07 ~ 538.20μg, r = 1.000 0. The recoveries were 98.0% (RSD = 1.2%). The linear range of baicalein was 1.12 ~ 111.60μg, r = 1.000 0, and the recoveries were 98.5% (RSD = 0.9% , r = 1.000 0, the recovery was 99.0% (RSD = 1.4%). Conclusion The method is simple, accurate, reproducible and highly specific and can be used for the quality control of the preparation.