论文部分内容阅读
Objective To examine whether ghrelin has beneficial effect on survival of pancreatic INS-1 beta cell.Methods Rat INS-1 cells were cultured separately in 5.6mmol/L glucose(NG group),33.3 mmol/L glucose(HG group),33.3 mmol/L glucose plus 10 nmol/L acylated ghrelin(HG+AG group),and 33.3 mmol/L glucose plus 10μmol/L unacylated ghrelin(HG+UG group).After being incubated for different hours,cell survival rate was determined by MTT.Activity of caspase-3 was estimated by spectrophotometry,activity
Objective To examine whether ghrelin has a beneficial effect on survival of pancreatic INS-1 beta cell. Methods Rat INS-1 cells were cultured separately in 5.6 mmol / L glucose (NG group), 33.3 mmol / L glucose (HG group) / L glucose plus 10 nmol / L acylated ghrelin (HG + AG group) and 33.3 mmol / L glucose plus 10 μmol / L unacylated ghrelin (HG + UG group). After being incubated for different hours, cell survival rate was determined by MTT . Activity of caspase-3 was estimated by spectrophotometry, activity