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以产黄青霉(Penicillium chrysogenum)PC-01为生产菌株,利用响应面法优化了青霉素合成的原始摇瓶发酵培养基配方。对原始发酵培养基中的6个成分进行析因试验,采用Minitab16软件分析显示玉米浆、乳糖和磷酸二氢钾这3个因素对青霉素发酵影响显著;将其他3个影响不显著的因子固定在适当水平,对上述3个显著影响因子进行最陡爬坡试验,确定了最优条件范围;再利用中心组合试验确定了最优条件。优化后的培养基配方为:玉米浆15.9 g/L、乳糖104.8 g/L、磷酸二氢钾5.5 g/L、硫酸铵2.5 g/L、硫酸钠0.5 g/L和碳酸钙5 g/L。在此条件下,青霉素的理论产量为16 996 u/ml,经验证,实际产量(17 101 u/ml)与预计产量相差0.62%,较初始青霉素产量(12 500 u/ml)提高了36.8%,降低了杂质组分的含量。
Using penicillium chrysogenum PC-01 as production strain, the response surface methodology was used to optimize the original shake flask fermentation medium for penicillin synthesis. Six factors of the original fermentation medium were analyzed by factorial analysis. The results of Minitab16 software analysis showed that three factors, corn steep liquor, lactose and potassium dihydrogen phosphate, had a significant effect on penicillin fermentation. The other three factors with insignificant effects were fixed at At the appropriate level, the steepest ascent test was carried out on the above three significant influencing factors to determine the optimal range of conditions. The optimum conditions were determined by the center combination test. The optimum medium was 15.9 g / L of corn syrup, 104.8 g / L of lactose, 5.5 g / L of potassium dihydrogen phosphate, 2.5 g / L of ammonium sulfate, 0.5 g / L of sodium sulfate and 5 g / L of calcium carbonate . Under these conditions, the theoretical yield of penicillin was 16 996 u / ml. The actual yield (17 101 u / ml) was 0.62% of the expected yield, 36.8% more than the initial yield of penicillin (12 500 u / ml) , Reducing the content of impurity components.