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目的 探讨新疆维吾尔族子宫颈癌端粒酶的活性。方法 采用 TRAP- PCR- EL ISA法和 TRAP- PCR-电泳法 ,对新疆维吾尔族子宫颈癌组织和正常组织进行了检测。结果 子宫颈癌组织中端粒酶活性的阳性检出率为 88.2 % ,而正常子宫颈组织中为 9.5 % ,癌组织较正常组织端粒酶活性明显增高。结论 子宫颈癌各病理指标临床分期均可检测出端粒酶活性 ,此现象反映了癌发展过程中的早期变化。同其他民族的子宫颈癌研究结果相同。端粒酶活性在维吾尔族子宫颈癌中也表现增高 ,因此 ,也可作为该民族子宫颈癌的一个标志物。另外 ,用 TRAP- PCR-EL ISA较 TRAP- PCR电泳法敏感性强。其发病机制有待进一步的研究
Objective To investigate the telomerase activity of Uygur cervical cancer in Xinjiang Uygur Autonomous Region. Methods The Uygur cervical cancer tissues and normal tissues were detected by TRAP-PCR-ELISA and TRAP-PCR-electrophoresis. Results The positive rate of telomerase activity in cervical cancer tissues was 88.2%, while that in normal cervical tissues was 9.5%. The telomerase activity in cancer tissues was significantly higher than that in normal tissues. Conclusion The telomerase activity can be detected in clinical stages of cervical cancer. This phenomenon reflects the early changes in the development of cancer. The same with other ethnic cervical cancer research results. Telomerase activity is also increased in Uighur cervical cancer and therefore can be used as a marker of cervical cancer in the nation. In addition, TRAP-PCR-EL ISA is more sensitive than TRAP-PCR. Its pathogenesis needs further study