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目的探讨表阿霉素(EPI)诱导肝癌细胞与乳腺癌细胞凋亡的作用,为临床治疗提供依据。方法采用流式细胞术、形态学方法观察EPI对肝癌细胞SMMC-7721与乳腺癌细胞MD-231的促凋亡作用。结果EPI在体外诱导SMMC-7721和MD-231细胞凋亡,经激光共聚焦显微镜检测,细胞呈现典型的凋亡形态特征;流式细胞术分析显示EPI(10μg/ml)作用两种细胞12h后,S期细胞明显增加,与对照组比较差异有高度统计学意义(P<0.01),表明细胞阻滞于S期。结论EPI具有诱导肝癌细胞和乳腺癌细胞凋亡的作用。
Objective To investigate the role of epirubicin (EPI) in inducing apoptosis of hepatocellular carcinoma cells and breast cancer cells and provide evidence for clinical treatment. Methods Flow cytometry and morphological methods were used to observe the effect of EPI on the apoptosis of hepatocellular carcinoma cell line SMMC-7721 and breast cancer cell line MD-231. Results The apoptosis of SMMC-7721 and MD-231 cells was induced by EPI in vitro. The cells were characterized by laser scanning confocal microscope. The results of flow cytometry showed that EPI (10μg / ml) , S phase cells increased significantly, compared with the control group, the difference was statistically significant (P <0.01), indicating that cells arrested in the S phase. Conclusion EPI can induce the apoptosis of hepatoma cells and breast cancer cells.