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材料和方法:取Sprague Dawley雄性大鼠(体重2 0 0～2 5 0g) 2 4只,制作糖尿病模型[1] ,随机分成糖尿病组(DM组)和治疗组,每组再分为2周、6个月组,每组6只。鼠龄配对的12只正常雄性大鼠为对照组。治疗组在造模后立即应用神经生长因子(NGF)后肢肌肉注射80 0U·kg-1·d-1。2周及6?
MATERIALS AND METHODS: Twenty four Sprague Dawley male rats weighing 200-250 g were used to make a diabetic model [1]. They were randomly divided into diabetic group (DM group) and treatment group, and each group was divided into two groups , 6 months group, 6 rats in each group. Twelve normal male rats were used as controls. The treatment group immediately after modeling using nerve growth factor (NGF) hind limb muscle injection 80 0U · kg-1 · d-1.2 weeks and 6?