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目的 探讨抗氧化剂 -过氧化氢酶、还原型谷胱甘肽和 β -胡萝卜素是否对紫外线照射诱发的DNA损伤具有拮抗作用。方法 提取人外周血中的淋巴细胞 ,用单细胞凝胶电泳法检测经不同浓度过氧化氢酶、还原型谷胱甘肽或 β -胡萝卜素处理及应用不同计量紫外线照射后淋巴细胞的DNA损伤状况。 结果 随着紫外线照射剂量的增加 ,淋巴细胞的DNA损伤程度逐渐加重 ;外源性过氧化氢酶、还原型谷胱甘肽和 β-胡萝卜素的加入可明显减轻DNA损伤程度 ,但均未能完全阻断DNA的损伤 ;在一定范围内 ,这些抗氧化剂的剂量与拮抗功效呈量效关系。结论 过氧化氢酶、还原型谷胱甘肽和β 胡萝卜素对紫外线照射诱发的DNA损伤均有拮抗作用 ,是拮抗紫外线照射诱发DNA损伤较为理想的拮抗剂 ;单细胞凝胶电泳法适用于研究紫外线照射引起的DNA损伤。
Objective To investigate whether antioxidants - catalase, reduced glutathione and β - carotene have antagonistic effects on DNA damage induced by ultraviolet radiation. Methods Lymphocytes from human peripheral blood were extracted and single cell gel electrophoresis was used to detect DNA damage of lymphocytes after treated with different concentrations of catalase, reduced glutathione or β - carotene and with different doses of ultraviolet light situation. Results With the increase of UV dose, the degree of DNA damage in lymphocytes gradually increased. Exogenous catalase, reduced glutathione and β-carotene could significantly reduce DNA damage, but none of them failed Completely blocked the DNA damage; within a certain range, the dose of these antioxidants and antagonistic efficacy dose-effect relationship. Conclusions Catalase, reduced glutathione and β-carotene have antagonistic effects on DNA damage induced by ultraviolet radiation and are ideal antagonists against DNA damage induced by UV irradiation. Single cell gel electrophoresis is suitable for studying DNA damage caused by UV radiation.