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目的:建立HPLC测定增智营养片中牛磺酸及盐酸赖氨酸的含量。方法:用2,4-二硝基氟苯柱前衍生化,采用Inertsil C18色谱柱,乙腈-磷酸盐缓冲溶液(19:81)为流动相,检测波长360nm进行测定。结果:牛磺酸及盐酸赖氨酸的线性范围分别为(0.005～0.05)mg.mL-1,r=0.9999,(0.01～0.1)mg.mL-1,r=0.9998,平均回收率分别为99.1%和98.6%,RSD值分别为2.14%和2.06%。结论:该方法操作相对简单,专属性强,重复性好,准确性高,可用于本品的质量控制。
Objective: To establish a HPLC method for the determination of taurine and lysine hydrochloride in Zengzhi nourishing tablets. METHODS: Pre-derivatization with 2,4-dinitrofluorobenzene was performed on an Inertsil C18 column with acetonitrile-phosphate buffer (19:81) as the mobile phase at a detection wavelength of 360 nm. Results: The linear ranges of taurine and lysine hydrochloride were (0.005-0.05) mg.mL-1, r = 0.9999, (0.01-0.1) mg.mL-1 and r = 0.9998.The average recoveries were 99.1% and 98.6% respectively, with RSDs of 2.14% and 2.06% respectively. Conclusion: The method is relatively simple, specific, reproducible, accurate and can be used for the quality control of this product.