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目的:建立清火栀麦片中穿心莲内酯和脱水穿心莲内酯的含量测定方法。方法:以XTerra RP18色谱柱为分离柱,以甲醇-水(45:55)为流动相,采用SPE-HPLC法测定。结果:本品中穿心莲内酯含量测定线性范围为(16.08～160.8)μg.mL-1(r=0.9999),平均回收率为98.40%(RSD=1.98%,n=6);脱水穿心莲内酯含量测定线性范围为(8.208～82.08)μg.mL-1(r=0.9999),平均回收率为97.44%(RSD=1.31%,n=6)。结论:此法操作简单、结果准确,可用于清火栀麦片的质量控制。
Objective: To establish a method for the determination of andrographolide and dehydroandrographolide in Qinghuo buckwheat tablets. Methods: XTerra RP18 column was used as the separation column and the methanol-water (45:55) mobile phase was used for the determination by SPE-HPLC. Results: The linear range of andrographolide content in this product was (16.08～160.8)μg.mL-1 (r=0.9999), the average recovery was 98.40% (RSD=1.98%, n=6); dehydroandrographolide The linear range of the assay was (8.208-82.08) μg.mL-1 (r=0.9999) and the average recovery was 97.44% (RSD=1.31%, n=6). Conclusion: This method is simple, accurate, and can be used to control the quality of clear fire buckwheat.