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酶消化法获得原代培养的牙髓干细胞(dental pulp stem cells,DPSCs),有限稀释法进行克隆培养。取3代DPSCs,以不同的培养条件将其分为5个大组,用含bFGF(5ng/mL)的培养液和矿化液为实验组,不含bFGF的培养液和矿化液为对照组,以不同培养条件培养DPSC后,用茜素红定量法对其矿化能力进行检测,分析不同培养条件对DPSC矿化能力的影响。结果:含bFGF的培养液预培养DPSCs1周后其矿化能力增强(P<0.05),2周后其矿化能力减弱(P<0.05);
Primary cultured dental pulp stem cells (DPSCs) were obtained by enzymatic digestion and cloned by limiting dilution. Take 3rd generation DPSCs, which were divided into 5 groups according to different culture conditions. The culture medium and mineralized solution containing bFGF (5ng / mL) were used as the experimental group, the medium without bFGF and the mineralized solution as control Group. DPSC was cultured under different culture conditions. The mineralized ability of DPSC was assayed by alizarin red quantitative method. The effects of different culture conditions on the mineralization ability of DPSC were analyzed. Results: After preincubated with bFGF for 1 week, the mineralization ability of DPSCs was enhanced (P <0.05). After 2 weeks, the mineralization ability of DPSCs was weakened (P <0.05).