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目的探索鸡和疣鼻栖鸭Ia-相关的恒定链(CIi和MDIi)的交叉免疫原性。方法用间接ELISA检测抗CIi小鼠血清与原核表达MDIi的反应性、抗MDIi小鼠血清与原核表达CIi的反应性;通过荧光免疫组织化学法鉴定抗CIi小鼠血清与疣鼻栖鸭脾组织MDIi的荧光反应强度、抗MDIi小鼠血清与鸡肝组织CIi的荧光反应强度。结果抗MDIi小鼠血清与原核表达CIi的反应滴度为1∶8000,抗CIi小鼠血清与原核表达MDIi的反应滴度为1∶16 000;采用抗MDIi小鼠血清、抗CIi小鼠血清进行免疫荧光组织化学染色,均能检测异种鸟类组织内的Ii抗原,但荧光染色强度均弱于同种鸟类Ii抗原组织的荧光反应强度。结论 CIi和MDIi具有交叉免疫原性,CIi和MDIi的序列保守性是形成共同抗原表位的分子基础,从免疫学角度揭示了鸟类Ii的高度保守性。
Objective To explore the cross-immunogenicity of Ia-related constant chains (CIi and MDIi) in chickens and Cairines. Methods The reactivity of anti-CIi mice sera with prokaryotic MDIi and the reactivity of anti-MDIi mice sera with prokaryotic expression CIi were detected by indirect ELISA. The anti-CIi mice sera were identified by fluorescence immunohistochemistry. The fluorescence intensity of MDIi, the fluorescence intensity of anti-MDIi mouse serum and chicken liver tissue CIi. Results The titer of anti-MDIi mouse serum was 1: 8000 with the prokaryotic expression of CIi, the titer of anti-CIi mouse serum with prokaryotic MDIi was 1:16 000, the anti-MDIi mouse serum, anti-CIi mouse serum Immunofluorescence histochemical staining, can detect Ii antigen in different species of birds, but the intensity of fluorescence staining is weaker than that of the same species of bird Ii antigen fluorescence intensity. Conclusion CIi and MDIi have cross-immunogenicity. The sequence conservation of CIi and MDIi is the molecular basis for the formation of common epitopes. From the perspective of immunology, Ii is highly conserved.