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采用固相萃取(SPE)-高效液相色谱法(HPLC)检测羊肉风干肠中色胺、尸胺、组胺、酪胺、亚精胺和精胺6种生物胺(BAs)。对比了液液萃取和采用不同萃取柱(ODS-C18萃取柱、阳离子SCX萃取柱、阴离子萃取SAX柱)进行固相萃取对样品中生物胺的净化效果。通过研究发现,固相萃取法对生物胺具有较好的净化、富集作用,其中强阳离子SCX萃取柱效果最佳,生物胺线性回归范围在0.5~25 mg/L,方法的检出限在0.01~0.1 mg/L之间,定量限在0.1~1.0 mg/L之间。羊肉风干肠中加标回收率为74.25%~98.37%,相对标准偏差(RSD,n=6)为9.36%~17.27%,方法的准确性和回收率较高,可以有效检测羊肉风干肠中生物胺含量。
Six kinds of biogenic amines (BAs), such as tryptamine, cadaverine, histamine, tyramine, spermidine and spermine, were detected by SPE and HPLC. The purification of biogenic amines in liquid samples by liquid-liquid extraction and solid-phase extraction using different extraction columns (ODS-C18, cation SCX and anion SAX) was compared. The results showed that the solid phase extraction method had a good purification and enrichment effect on biogenic amines. Among them, the strong cation SCX extraction column was the best, the linear range of biogenic amines was in the range of 0.5-25 mg / L, and the detection limit was 0.01 ~ 0.1 mg / L, the limit of quantification is 0.1 ~ 1.0 mg / L. The recoveries of mutton in dried sausage of lamb were 74.25% ~ 98.37%, the relative standard deviations (RSDs, n = 6) were 9.36% ~ 17.27%. The accuracy and recovery of the method were high. Amine content.