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以合丰23和合丰35的子叶节和胚尖为外植体,通过农杆菌介导对抗大豆疫霉根腐病基因SR1进行遗传转化。结果表明:合丰35胚尖和子叶节体系的出芽率(96.1%和79.1%)均显著高于合丰25(66.45%和74.4%);胚尖转化体系的平均再生周期(40 d)低于子叶节转化体系(68 d);在诱导培养基上培养20 d时胚尖转化体系的转化效率(96.1%)高于子叶节体系(77.8%)。以合丰35胚尖为外植体成功转化大豆抗疫霉根腐病基因SR1,共获得6株转基因植株。
The cotyledonary node and embryo tips of Hefeng 23 and Hefeng 35 were used as explants to transform the SR1 gene of soybean by Phytophthora capsici. The results showed that the germination rates of Hefeng 35 and Cotyledonary node systems (96.1% and 79.1%) were significantly higher than those of Hefeng 25 (66.45% and 74.4%), respectively. The mean regeneration cycle (40 days) In the cotyledonary node transformation system (68 d), the transformation efficiency (96.1%) of the embryogenic transformation system was higher than that of the cotyledonary node system (77.8%) when cultured on the induction medium for 20 days. Transgenic plantlets of Hefeng 35 were successfully transformed into SR1, a total of 6 transgenic plants.