论文部分内容阅读
【目的】解决樱桃砧木吉赛拉组培快繁生产中玻璃化苗发生及污染问题。【方法】通过对培养基中激素、蔗糖、琼脂浓度的调节及培养温度的控制,防止玻璃化苗发生;通过培养基中加入杀菌剂,寻求解决组培苗污染。【结果】培养基中细胞分裂素6-BA的浓度控制在1 mg/L以下、蔗糖浓度40 g/L、琼脂浓度6 g/L左右、培养温度24℃左右,可有效地预防玻璃化苗的发生;在培养基中加入500～1 000 mg/L多菌灵50%可湿性粉剂,可有效防止污染的发生。【结论】在樱桃砧木吉赛拉5号组培快繁中,细胞分裂素6-BA浓度、蔗糖浓度、琼脂浓度、培养温度对试管苗产生玻璃苗都有一定的影响,其中,细胞分裂素6-BA浓度、培养温度的影响更为明显。为了防止污染的发生,培养基中添加多菌灵50%可湿性粉剂效果较好。
【Objective】 The objective of this study was to solve the problem of vitrification and pollution in the rapid propagation of tissue culture of cherry rootstocks. 【Method】 Vitrification was prevented by regulating the concentration of hormones, sucrose and agar in culture medium and controlling the culture temperature. The disinfection of tissue culture seedlings was attempted by adding bactericide in culture medium. 【Result】 The results showed that the concentration of cytokinin 6-BA in the medium was controlled below 1 mg / L, the concentration of sucrose was 40 g / L, the concentration of agar was about 6 g / L and the culture temperature was about 24 ℃. Of the occurrence; in the medium by adding 500 ~ 1 000 mg / L carbendazim 50% WP, can effectively prevent the occurrence of pollution. 【Conclusion】 Cytokinin 6-BA concentration, sucrose concentration, agar concentration and culture temperature have certain effects on the production of glass seedlings in vitro. 6-BA concentration, the impact of incubation temperature is more obvious. In order to prevent the occurrence of pollution, the carbendazim 50% wettable powder added better effect.