论文部分内容阅读
目的观察不同浓度姜黄素对慢性阻塞性肺疾病(COPD)患者外周血内皮祖细胞(EPCs)数量、增殖功能、一氧化氮(NO)分泌功能及内皮型一氧化氮合酶(e NOS)表达的影响,探讨姜黄素修复和保护COPD患者受损的血管内皮功能作用的可能机制。方法选择30例住院的COPD患者,密度梯度离心法获取外周血单个核细胞,加入不同浓度姜黄素(0、5、10、15μmol·L-1)刺激并诱导其分化为EPCs,荧光双染色鉴定EPCs并进行细胞计数。收集贴壁细胞,再加入各浓度姜黄素,相同条件下培养一定时间(6、12、24、48 h)。采用CCK-8法检测EPCs的增殖能力,硝酸还原酶法(Griess法)检测NO分泌量,Western Blot法检测e NOS蛋白的表达。结果与未加入姜黄素的对照组相比,姜黄素可显著增加COPD患者EPCs的数量,并呈浓度依赖性;姜黄素显著改善了EPCs的增殖功能及NO分泌功能,呈浓度时间依赖性;姜黄素也可显著上调EPCs的e NOS表达,与姜黄素浓度之间具有相关性。结论姜黄素增加了EPCs的数量,改善了EPCs增殖功能及NO分泌功能,并上调了e NOS的表达。提示姜黄素可能通过增加EPCs的数量及改善EPCs的功能来修复COPD患者的血管内皮功能损伤。
Objective To observe the effects of curcumin on EPCs, proliferation, nitric oxide (NO) secretion and endothelial nitric oxide synthase (eNOS) expression in peripheral blood of patients with chronic obstructive pulmonary disease (COPD) To investigate the possible mechanism of curcumin repair and protection of impaired endothelial function in COPD patients. Methods Thirty patients with COPD were enrolled in this study. Peripheral blood mononuclear cells were obtained by density gradient centrifugation. The cells were stimulated with different concentrations of curcumin (0, 5, 10, 15μmol·L-1) and induced to differentiate into EPCs. EPCs and cell count. Adherent cells were collected, and then added with curcumin at the same concentration for a certain period of time (6, 12, 24, 48 h). The proliferation of EPCs was detected by CCK-8 assay, NO secretion was detected by Griess method, and eNOS protein expression was detected by Western Blot. Results Compared with the control group without curcumin, curcumin significantly increased the number of EPCs in COPD patients in a concentration-dependent manner. Curcumin significantly improved the proliferation and NO secretion of EPCs in a concentration-dependent manner. Curcumin Su also significantly up-regulated the eNOS expression of EPCs, which was correlated with the concentration of curcumin. Conclusion Curcumin increases the number of EPCs, improves EPCs proliferation and NO secretion, and up-regulates eNOS expression. These results suggest that curcumin can repair vascular endothelial dysfunction in COPD patients by increasing the number of EPCs and improving the function of EPCs.