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建立了11种心血管保健功效成分(天麻素、吡啶甲酸铬、腺苷、大豆苷元、大豆苷、染料木苷、染料木素、红景天苷、葛根素、芦丁、淫羊藿苷)的反相高效液相色谱同时检测法,采用C18柱,在25℃,1 mL/min流速下,以甲醇-KH2PO4二元流动相梯度洗脱,二极管阵列检测器检测。在单因素实验基础上,采用响应曲面法对提取工艺进行了进一步优化。方法在25 min内实现基线分离,线性相关系数r≥0.9985,检出限范围为0.008~0.20μg/mL,日内精密度≤1.5%,日间精密度≤5.0%,回收率在85.0%~98.2%之间。
A total of 11 cardiovascular health effects were established (gastrodin, chromium picolinate, adenosine, daidzein, daidzin, genistin, genistein, salidroside, puerarin, rutin, icariin ) Reversed-phase high-performance liquid chromatography simultaneous detection method, C18 column, at 25 ℃, 1 mL / min flow rate with methanol - KH2PO4 binary mobile phase gradient elution, diode array detector detection. Based on the single factor experiment, the extraction process was further optimized by response surface methodology. Method was used to achieve the baseline separation in 25 min. The linear correlation coefficient was more than or equal to 0.9985 with the detection limit of 0.008-0.20 μg / mL, the intra-day precision of ≤ 1.5%, the intra-day precision of ≤ 5.0% and the recovery of 85.0% ~ 98.2 %between.