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目的研究外源水杨酸(SA)对盐(NaCl)胁迫下广藿香Pogostemon cablin种子萌发和幼苗生长的影响,并筛选缓解盐胁迫的SA最适浓度。方法用50 mmol/L NaCl溶液模拟盐胁迫,采用纸上发芽,研究不同浓度梯度外源SA处理对盐胁迫下广藿香种子的发芽势(GE)、发芽率(GR)、发芽指数(GI)、活力指数(VI)、苗长(SL)、根长(RL)、苗鲜质量(SFW)、苗干质量(SDW)、叶绿素量(SPAD值)、可溶性蛋白质(SP)量和丙二醛(MDA)量的变化,并对各指标进行相对盐害率分析。结果盐胁迫显著抑制了广藿香种子萌发和幼苗生长。0.10和0.25 mmol/L SA处理均可有效缓解广藿香盐害程度,而0.50 mmol/L SA处理却加剧了盐害程度。综合考虑各项指标,0.25 mmol/L SA处理的盐害缓解效果最好,与盐对照相比,GE、GR、GI、VI、SL、RL、SFW、SDW、SPAD值和SP量分别显著提高46.02%、27.45%、30.16%、92.63%、39.80%、47.94%、26.48%、18.85%、15.94%和14.70%,且叶片MDA量显著降低18.35%。结论一定浓度范围的SA可明显缓解盐胁迫对广藿香种子萌发和幼苗生长的抑制作用,提高广藿香种子的萌发能力和幼苗对环境的适应能力,以0.25mmol/L SA效果最佳,但过高质量浓度SA处理会加剧盐胁迫对广藿香的不利影响。
Aim To investigate the effects of exogenous salicylic acid (SA) on the seed germination and seedling growth of Pogostemon cablin under salt (NaCl) stress and to screen for the optimum SA concentration for salt stress. Methods Salt stress was induced in 50 mmol / L NaCl solution and germinated on paper to study the effects of different concentrations of exogenous SA on the germination potential (GE), germination rate (GR), germination index ), Vitality index (VI), SL, RL, SFW, SDW, SPAD, SP, Aldehyde (MDA) content changes, and each index relative salt damage rate analysis. Results Salt stress significantly inhibited seed germination and seedling growth of patchouli. Both 0.10 and 0.25 mmol / L SA treatment could effectively alleviate the damage of patchouli salt, while 0.50 mmol / L SA treatment aggravated the degree of salt damage. Considering all the indexes, salt treatment with 0.25 mmol / L SA had the best alleviation effect. Compared with salt control, the contents of GE, GR, GI, VI, SL, RL, SFW, SDW, SPAD and SP were significantly increased 46.02%, 27.45%, 30.16%, 92.63%, 39.80%, 47.94%, 26.48%, 18.85%, 15.94% and 14.70%, respectively. Conclusion SA at a certain concentration range can obviously alleviate the inhibitory effect of salt stress on the seed germination and seedling growth of patchouli, increase the germination ability of patchouli seed and the adaptability of seedling to environment. The effect of 0.25 mmol / L SA is the best, But too high concentration of SA could aggravate the adverse effects of salt stress on patchouli.