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目的:观察氯吡格雷对兔动脉粥样硬化(AS)血清基质金属蛋白酶-9(MMP-9)含量、血管壁核因子-κB/p65(NF-κB/p65)含量 mRNA表达的影响。方法:将27只新西兰雄性白兔随机分为正常对照组(A组)、高胆固醇组(B组)和氯吡格雷组(C组)。酶法检测血脂;原位杂交法检测血管壁NF-κB/p65 mRNA表达;固相酶联免疫吸附试验(ELISA)检测MMP-9及血管壁NF-ΚB/p65含量;组织形态学分析AS斑块/内膜面积比值及斑块最厚处内膜/中膜厚度比值。结果:B组和C组血清MMP-9、血管壁NF-κB/p65含量及 mRNA表达均较A组显著升高(P<0.05);C组较B组AS病变明显减轻(P<0.05),MMP-9、血管壁NF-κB/p65含量及 mRNA及p65表达均明显降低(均P<0.05)。结论:氯吡格雷可能具有一定抗AS作用,且抗AS作用机制可能与抑制NF-κB/p65及MMP-9表达有关。
Objective: To observe the effect of clopidogrel on serum MMP-9 and the mRNA expression of NF-κB / p65 in atherosclerotic rabbits. Methods: 27 male New Zealand white rabbits were randomly divided into normal control group (A group), high cholesterol group (B group) and clopidogrel group (C group). The levels of MMP-9 and NF-κB / p65 in the vascular wall were detected by enzyme-linked immunosorbent assay (ELISA) and histopathological analysis Block / intima area ratio and the thickness of the plaque intima / media thickness ratio. Results: The contents of MMP-9, NF-κB / p65 and the mRNA expression of vascular wall in group B and C were significantly higher than those in group A (P <0.05) , MMP-9, NF-κB / p65 and the mRNA and p65 expression of vascular wall were significantly decreased (all P <0.05). Conclusion: Clopidogrel may have some anti-AS effects, and the anti-AS mechanism may be related to the inhibition of the expression of NF-κB / p65 and MMP-9.