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目的研究改造后的血管生成抑制相关肽(21肽)抗血管生成活性及对人宫颈癌Hela细胞皮下移植裸鼠模型的抑制作用,探讨其抑瘤作用机制。方法2005年8月至2006年2月哈尔滨医科大学第一临床医学院进行Hela细胞株皮下接种裸鼠造模,21肽治疗3周后,处死动物剥离肿瘤,称重并计算抑瘤率。免疫组化方法检测21肽对肿瘤组织的微血管密度(MVD)、增殖细胞核抗原(PCNA)和血管内皮生长因子(VEGF)的影响。结果21肽组平均抑瘤率可达51.89%,肿瘤组织微血管密度明显降低,与对照组比较差异有统计学意义(P<0.05);21肽组肿瘤组织的PCNA、VEGF呈低表达,与对照组比较差异有统计学意义(P<0.05)。结论21肽具有显著的抗血管生成活性,可明显地抑制裸鼠宫颈癌细胞的生长,其抑制宫颈癌的作用机制可能与其降低新生血管形成和调解血管生成相关因子的表达有关。
Objective To study the anti-angiogenic activity of the modified angiogenesis inhibitory peptide (21 peptide) and its inhibitory effect on the subcutaneous transplantation of human cervical cancer Hela cells in nude mice and to explore the mechanism of its anti-tumor effect. Methods From August 2005 to February 2006, Hela cell lines were inoculated subcutaneously in nude mice from Harbin Medical University No.1 School of Clinical Medicine. After 21 weeks of treatment with 21 peptide, the animals were sacrificed and the animals were sacrificed and weighed to calculate the tumor inhibition rate. Immunohistochemistry was used to detect the effect of 21 peptide on the microvessel density (MVD), proliferating cell nuclear antigen (PCNA) and vascular endothelial growth factor (VEGF) in tumor tissue. Results The average tumor inhibitory rate of 21 peptide group was 51.89%, the microvessel density of tumor tissue was significantly lower than that of control group (P <0.05). The expression of PCNA and VEGF in 21 peptide group was lower than that of control group The difference was statistically significant (P <0.05). Conclusions 21 peptide has significant anti-angiogenic activity and can obviously inhibit the growth of cervical cancer cells in nude mice. The mechanism of its inhibitory effect on cervical cancer may be related to the decrease of angiogenesis and the regulation of angiogenesis-related factors.