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本实验采用人工胸膜再造及荧光素钠血管内造影的方法观察了大鼠活体肺表面的微循环及其运动反应并同时进行连续摄像录像分析。这种肺微循环的观察方法能清楚地判断肺血流方向,区分肺微动、静脉,而且各级血管显影清晰、边界明确,能准确地测量微血管的真实口径变化及其对刺激的反应与动态改变。血管紧张素Ⅱ收缩管径40μm以上的肺微动脉,去甲肾上腺素既收缩肺微动脉,又收缩微静脉,而血小板激活因子则轻度扩张肺微动脉,急性肺泡缺氧亦可致肺微动脉及微静脉收缩。这些结果提示:肺微血管对循环中的活性物质及肺泡缺氧有明显的反应,这种反应可能在通气/血流比值调节方面起重要作用。该实验模型可用于肺微循环的调节及某些药物和致病因素作用的研究。
In this study, artificial pleural reconstruction and intravascular fluorescein angiography methods were used to observe the microcirculation and motor response of the living lung surface of rats and to conduct continuous video recording analysis. This observation of pulmonary microcirculation can clearly determine the direction of pulmonary blood flow, pulmonary micro-distinguish between the vein, and at all levels of blood vessels clear, clear boundary, can accurately measure the real changes in microvascular and its response to stimulation and Dynamic change. Angiotensin Ⅱ systolic diameter of more than 40μm pulmonary arterioles, norepinephrine contraction of both pulmonary arterioles, contractions of venules, and platelet activating factor is mild expansion of pulmonary arterioles, acute alveolar hypoxia can also cause lung micro- Artery and venule contraction. These results suggest that pulmonary microvessels have a marked response to circulating substances and alveolar hypoxia, and this response may play an important role in ventilation / blood flow ratio regulation. The experimental model can be used for regulation of pulmonary microcirculation and the role of certain drugs and pathogenic factors.