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目的:观察复健片对脑梗死模型大鼠运动功能以及脑组织酪氨酸受体激酶B(trk-B)和勿动蛋白A(Nogo-A)表达的影响,探讨其促进神经再生作用机制。方法:90只Wistar大鼠随机分为药物组、模型组、假手术组,每组30只。用电凝法制备大脑中动脉闭塞大鼠模型,造模成功后6 h药物组灌胃给予复健片水溶液9 g.kg-1,其余2组分别灌胃给予同等量生理盐水,每天1次,共2周。用前肢放置检测法评定运动功能;分别用免疫组化法和原位杂交法观察脑组织trk-B和Nogo-A的表达,并测定染色平均灰度。结果:药物组大鼠运动功能评分(86.34±5.48)明显高于模型组(62.26±14.87),P<0.01;trk-B表达药物组(95.98±3.55)较模型组明显增强(110.10±6.82),P<0.01;而Nogo-A药物组(131.09±8.99)表达较模型组明显减弱(121.36±11.06),P<0.01。结论:复健片可显著增强trk-B并抑制Nogo-A的表达,从而改善神经再生微环境,促进脑梗死大鼠运动功能康复。
Objective: To observe the effect of rehabilitation tablet on motor function and the expression of tyrosine kinase B (Trk-B) and Nogo-A in cerebral infarction rats and to explore its mechanism of promoting nerve regeneration . Methods: Ninety Wistar rats were randomly divided into drug group, model group and sham operation group, 30 rats in each group. The middle cerebral artery occlusion rat model was established by electrocoagulation. After 6 h, the rats in the drug group were gavaged with 9 g.kg-1 of recuperative tablets and the other two groups were given the same amount of normal saline once a day , A total of 2 weeks. The forelimb placement test was used to evaluate the motor function. The expression of trk-B and Nogo-A in brain tissue was observed by immunohistochemistry and in situ hybridization, respectively. Results: Compared with the model group, the scores of motor function in the drug group were significantly higher than those in the model group (86.34 ± 5.48 vs 62.26 ± 14.87, P <0.01), and those in the trk-B group were significantly (110.10 ± 6.82) , P <0.01; while the Nogo-A drug group (131.09 ± 8.99) was significantly weaker than the model group (121.36 ± 11.06), P <0.01. Conclusion: Rehabilitation Tablet can significantly enhance trk-B and inhibit the expression of Nogo-A, so as to improve the microenvironment of nerve regeneration and promote the rehabilitation of motor function in rats with cerebral infarction.