迄今,已有报道证明,人体乳腺肿瘤衍生的细胞可能具有特异性的抗原,它可以使乳腺癌患者产生特异性抗体以及细胞介导的反应。然而,由于免疫反应的复杂性,这种抗血清的特异性很难检测。为了对肿瘤特异性抗原进行严格地分析,最好能获得一系列的单克隆抗体以鉴定其特异性。本研究即用激素依赖性的(hormone-dependent)人体乳腺癌细胞系2R-75-1免疫6-8周龄的雌性BALB/C小鼠,然后 So far, it has been reported that human breast tumor-derived cells may have specific antigens, which can produce specific antibody and cell-mediated response in breast cancer patients. However, the specificity of this antisera is difficult to detect due to the complexity of the immune response. For a rigorous analysis of tumor-specific antigens, it is best to obtain a series of monoclonal antibodies to identify their specificity. In this study, 6-8 week old female BALB / C mice were immunized with a hormone-dependent human breast cancer cell line 2R-75-1 and then