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目的:探讨活血化瘀药龙血竭在小鼠触须表皮修复中的作用。方法:4w龄雄性昆明小鼠,无菌条件下去除双侧触须部表皮,实验对照组、阳性药物对照组,龙血竭内服+外用组,每日观察创面结痂率,进行卡方检验。术后1d、3d、5d、7d取材,常规病理切片观察。结果:术后2d,三组结痂率差异有统计学意义(P=0.005),以龙血竭治疗组最高。术后1～3d,各组创面以炎性反应为主,龙血竭内服+外用组毛细血管通透性增高,炎细胞渗出较多,真皮层中成纤维细胞增生。术后5d,各组真皮层中成纤维细胞及胶原纤维合成和分泌增加;龙血竭内服+外用组炎细胞消失,可见较多新生毛细血管。术后7d,龙血竭内服+外用组新生表皮分层明显,真皮成纤维细胞及胶原纤维排列规则,表皮真皮连接紧密,基膜明显。结论:龙血竭在小鼠触须创面愈合早期能促进血痂尽早形成,抗炎作用明显,促进毛细血管增生,促进真皮及表皮细胞增殖。
Objective: To explore the role of blood stasis drug Dragon’s Blood in the repair of mouse tentacles epidermis. METHODS: Four-week-old male Kunming mice were treated with sterile epidermis to remove bilateral epiglottis epidermis, experimental control group, positive drug control group, and dragon blood spent internal or external use group. The rate of wound scarring was observed daily and the chi-square test was performed. After 1d, 3d, 5d, and 7d, materials were taken and observed by routine pathological sections. RESULTS: On the 2nd day after surgery, the difference in the crusting rate between the three groups was statistically significant (P=0.005), and the treatment group was the highest. From 1 to 3 days after operation, the inflammatory response was the main cause of the wounds in each group. The blood capillary permeability of the internal blood + external use group increased, the inflammatory cells bleed more, and fibroblasts proliferated in the dermis. At the 5th day after operation, the synthesis and secretion of fibroblasts and collagen fibers in the dermis were increased in all groups. The inflammatory cells in the internal or external use group of Dragon blood were disappeared, and more new capillaries were seen. At the 7th day after operation, the neonatal epidermal stratification was obvious in the internal or external use of Dragon’s blood. The arrangement of dermal fibroblasts and collagen fibers was regular. The epidermis was closely connected and the basement membrane was obvious. Conclusion: Dragon’s Blood can promote the early formation of blood stasis in the early stage of mouse beard-to-wound healing. It has obvious anti-inflammatory effect, promotes capillary proliferation, and promotes the proliferation of dermal and epidermal cells.