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以小麦生产品种小偃22、科农199和西农1376为受体品种,植物抗菌蛋白基因LTP作为目的基因,构建pAHC25-LTP表达载体,利用基因枪法转化小麦幼胚愈伤组织,获得转LTP基因T0代小麦1247株。经草铵膦(Phosphinothricin,PPT)抗性筛选及PCR分子检测,获得阳性再生植株209株,转化率1.87%。阳性再生植株接种条锈菌CYR29、CYR31和CYR32混合小种后进行抗条锈病鉴定,获得抗性植株74株,其中抗性显著提高的抗病植株11株。本研究初步证明抗菌蛋白基因LTP在小麦抗条锈病菌的侵染中有一定作用,为获得转抗菌蛋白基因的抗条锈病小麦品种奠定基础,以期获得育种中可应用的新型抗源材料。
The wheat Lactobacillus plantarum, Xiaoyan 22, Kornong 199 and Xinong 1376 were used as the recipient plants, and plant antimicrobial protein LTP was used as the target gene to construct pAHC25-LTP expression vector. The immature embryos were transformed by gene gun to obtain LTP Gene T0 wheat 1247 strains. After the screening of phosphinothricin (PPT) resistance and PCR, 209 regenerated plants were obtained with the conversion rate of 1.87%. The positive regenerated plants were inoculated with the hybrid races of CYR29, CYR31 and CYR32 to identify the resistance to stripe rust, and 74 resistant plants were obtained, in which 11 resistant plants with significantly improved resistance were obtained. This study proves that the antimicrobial protein gene LTP plays a role in the resistance of wheat to stripe rust resistance and lays a foundation for obtaining the stripe rust resistant wheat variety with anti-bacterial protein gene, in order to obtain the new anti-stroma material suitable for breeding.