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目的探讨快速引物原位标记技术检测鼻咽癌染色体异常的可行性。方法采用快速引物原位标记技术,分别以人3号和7号染色体特异性寡核苷酸作引物,检测15例鼻咽癌和5例鼻咽正常冰冻组织切片细胞染色体,染色体异常标准以标记信号≤1的细胞比例≥65%时视为染色体丢失,标记信号≥3的细胞比例≥6.5% 作为染色体拷贝数增加。结果鼻咽癌组织细胞3号染色体标记率为88.6%,10例(66.7%)染色体拷贝数增加;7号染色体标记率87.4%,5例(33.3%)染色体丢失;其中4例同时存在3号染色体拷贝数增加和7号染色体丢失。正常组织3号和7号染色体标记率分别为92%和91.8%,二倍体细胞分别为43.2%和43.6%,与鼻咽癌比较差异均有统计学意义(P<0.05),未发现三体细胞。结论快速引物原位标记技术可用于鼻咽癌冰冻组织切片中染色体的检测,染色体数目的改变可能有助于鼻咽癌的诊断。
Objective To investigate the feasibility of rapid primer in situ labeling for the detection of chromosomal abnormalities in nasopharyngeal carcinoma. Methods Rapid primer-in-situ labeling technique was used to detect the chromosomes of 15 nasopharyngeal carcinoma and 5 normal frozen sections of human nasopharynx, respectively, using primers 3 and 7 of human chromosome-specific oligonucleotide. The chromosomal abnormalities were marked by chromosomal abnormalities Chromosome loss was seen when the proportion of cells with signal ≤ 1 was ≥ 65%, and the proportion of cells with ≥3 marker signal was ≥6.5% as chromosome copy number increase. Results The chromosome number of chromosome 3 in nasopharyngeal carcinoma cells was 88.6%, and the chromosome copy number of 10 cases (66.7%) was increased. The chromosome markers on chromosome 7 were 87.4% and the chromosomes were missing in 5 cases (33.3%). Chromosomal copy number increase and chromosome 7 loss. The positive rates of chromosome 3 and chromosome 7 in normal tissues were 92% and 91.8%, respectively, and the diploid cells were 43.2% and 43.6%, respectively, which were significantly different from that of nasopharyngeal carcinoma (P <0.05) Somatic cells. Conclusion The rapid primer in situ labeling technique can be used for the detection of chromosomes in frozen tissue sections of nasopharyngeal carcinoma. The change of chromosome number may be helpful for the diagnosis of nasopharyngeal carcinoma.